Table Ix1.  Homology comparisons of Ixodes scapularis gene sets

  Ixosc19Evigene = Evigene 2019 RNA assembly (3 SRA projects)

  Ixosc14Evigene = Evigene 2014 RNA assembly (1 SRA project)

  Ixosc19NCBI    = NCBI 2019 genome gene models (Ixosc19NCBI) 

a. Ixodes scapularis gene sets aligned to Ixodes ricinis genes

Reference Ixodes ricinis (Ixoric18TSA) coding sequences, NCBI TSA ID GFVZ01000000, and conserved arthropod genes (BUSCO v9, n=1066).   Ixodes ricinis coding sequences found=64389 among all gene sets, npaired=31879

b. Scorpion proteins x Ixodes tick gene sets

Reference Centruroides sculpturatus (bark scorpion) of NCBI DEC-2017, GCF_000671375.1_Cexi_2.0;   Scorpion genes found=20736, of total=24591, main isoform

c. Human proteins x Ixodes tick gene sets

Reference Homo sapiens proteins, NCBI 2018, GCF_000001405.38_GRCh38.p12, Human genes found=14860, of total=20191, main isoform

c. Daphnia proteins x Ixodes tick gene sets

Reference Daphnia pulex proteins of EvidentialGene NOV-2017, doi:NNNNN, Daphnia genes found=15351, of total=32989, main isoform

Brief methods: 

   Ix1a. CDS-align with  "blastn -query ref.cds -db test3sets.cds -task dc-megablast -template_type coding",  score best align per test set, count hits/set (Found),   Best/set as any set w/in 1 bp of top match, and average percent aligned to ref CDS.  pairAln% is percent align where both sets matched ref.  Arthropod conserved proteins are measured with Hmmer hmmscan and BUSCO software.

  Ix1b,c,d. Protein align with blastp -query ref.aa -db test3sets.aa,  score best align per test gene set, count hits/set (Found),  Best/set as any set w/in 1 aa of top match, and average align (aa)/set.