Gene lola* is notable: it has the most alternate-introns found in any gene, just as for Nasonia jewel wasp, with 55 found alt introns spread over 200Kb in 1kb steps (more expected, Nasonia has 180+). If you study bee/wasp/ant social behavior, lola alts guide brain axon growth, remember that name: El-oh-el-aye Lola, lo lo lo lo Lola * http://www.ncbi.nlm.nih.gov/pmc/articles/PMC218142/ Nasonia Jewel Wasp, the gene with most abundant alternate intron splicing is lola, sharing 1 common splice point. All are 3' alternates, sharing common 5' exons. eg. xxxxx ... yyy xxxxx ........ zzz xxxxx .............. aaa ^ common hub exon/intron splice point These are found with RNA-seq mapping to genome. Location SCAFFOLD2:4430867:- n=180 = lola, or Longitudinals lacking protein, Gene id Nasvi2EG036900, alt t1 .. t86 , n=86 annotated alternates, with 180 alternate introns found in RNA-seq mapping to same splice point. Separation of lola hub-3' introns: median distance = 1048 bases, max=5083, min=511 hub intron minimum, maximum span: 10457 .. 301261 This fairly regular spacing over 300 Kb span is suggestive of more unfound splice variants at each 1kb step, up to ~300 splice variants. Apis mellifera honey bee has similar lola alternate introns from hub intron splice point, Location Group9_12:148988:+, Gene id amel45:GB53441, matches nasonia OGSv2.0:Nasvi2EG036900 In evg3hbee EvidentialGene mRNA assembly, 55 such hub-alternate introns are found. More probably exists in un-assembled reads. These span about 180 Kb in Apis, versus 300 Kb in Nasonia. Note also the recent 2014 NCBI Gnomon/Eukpipe gene annotation for honey bee finds the same lola hub intron with ~55 alternates. However about 20 have a bit different 3' splice than Evigene's gene assembly, again suggesting unfound alts. http://arthropods.eugenes.org/EvidentialGene/arthropods/nasoniawasp/genes/lola/ http://arthropods.eugenes.org/EvidentialGene/arthropods/honeybee/evg3hbee/lola/