Accurate mosquito genes reconstructed with EvidentialGene

	Aedes Scaffold 8 (scontig1.8) example map, with 3 gene sets (EvidentialGene = green, VectorBase = orange, VT16 = purple)	Aedes aeg. supercontig1.859 site of Oxitec GMO rDNA Oxitec reports that "..no genes appear to be disrupted by the OX513 rDNA construct insertion". Evigene construction finds several unreported genes here, possibly non-coding. US FDA, 2016, Oxitec Mosquito: GMO Aedes aegypti OX513A

Accurate mosquito gene example maps

Gene alignment statistics and maps are listed, Aedes aeg. on left, Anopheles fun. on right. For each table row, the reference gene of Drosophila mel. (RefGene), with aa size (RefSize) is given, then the best aligned EvidentialGene ID, and alignment size, best VectorBase (VBgene), and best other (VOgene, published Aedes Trinity-PASA or Anofun-Trinity). Differences of alignment for Evigene-VBgene (DiffVB) and Evigene-VOgene (DiffVO) are given.
-- Don Gilbert, 2016 June.

RefGene RefSize Evigene DiffVB VBgene DiffVO VOgene Name

dmel:NP_651397.1 189 Aedesg12bEVm012828t6,191 191 0,0 191 0,0 Mediator of RNA polymerase II transcription subunit 28

dmel:NP_651397.1 189 Anofunz4kEVm009865t1,114 0 AFUN008979-PA,114 0 anofun2trinc16010g1t1,114 Mediator of RNA polymerase II transcription subunit 28

; Alignment improvements by EvidentialGene
for 27 subunit genes of
Mediator of RNA polymerase II transcription
Table of Mediator RpII genes

dmel:NP_608968.2 3489 Aedesg12bEVm000060t1,3480 0 AAEL009823-PA,3480 0 pub16AAEL009823-PA,3480 Blue cheese

dmel:NP_608968.2 3489 Anofunz4kEVm000064t1,3501 2827 AFUN004205-PA,674 1513 anofun2trinc16509g10t1,1988 Blue cheese

;

dmel:NP_650943.1 286 Aedesg12bEVm006118t4,285 7 AAEL004548-PA,278 0 pub16AAEL004548-PA,285 Bride of doubletime

dmel:NP_650943.1 286 Anofunz4kEVm008697t1,286 286 0,0 286 0,0 Bride of doubletime

;

dmel:NP_477497.1 5147 Aedesg12bEVm000016t1,4500 4253 AAEL000700-PA,247 1227 pub16novel_model_1167.2.1,3273 Cadherin-related tumor suppressor

dmel:NP_477497.1 5147 Anofunz4kEVm000015t2,5247 1777 AFUN003587-PA,3470 0 anofun2trinc17282g1t1,5247 Cadherin-related tumor suppressor

;

dmel:NP_612019.2 1773 Aedesg12bEVm000119t7,1838 1838 0,0 1838 0,0 Disco-interacting protein 2

dmel:NP_612019.2 1773 Anofunz4kEVm000088t2,1869 94 AFUN007112-PA,1775 1869 0,0 Disco-interacting protein 2

;

RefGene RefSize Evigene DiffVB VBgene DiffVO VOgene Name

dmel:NP_001260032.1 22949 Aedesg12bEVm000003t1,11411 5552 AAEL012910-PA,5859 5552 pub16AAEL012910-PA,5859 Dumpy, isoform Q

dmel:NP_001260032.1 22949 Anofunz4kEVm000003t5,10169 4004 AFUN000137-PA,6165 7104 anofun2trinc18529g1t4,3065 Dumpy, isoform Q

;

dmel:NP_001261188.1 2761 Aedesg12bEVm000080t1,2807 -101 AAEL015015-PA,2908 -101 pub16AAEL015015-PA,2908 enhancer of bithorax, isoform H

dmel:NP_001261188.1 2761 Anofunz4kEVm000075t7,2458 1792 AFUN008674-PA,666 788 anofun2trinc19016g1t2,1670 enhancer of bithorax, isoform H

;

dmel:NP_524106.3 418 Aedesg12bEVm008096t2,329 54 AAEL003453-PA,275 54 pub16AAEL003453-PA,275 Failed axon connections

dmel:NP_524106.3 418 Anofunz4kEVm006171t1,336 72 AFUN007110-PA,264 0 anofun2trinc16131g4t1,336 Failed axon connections

;

dmel:NP_524191.1 412 Aedesg12bEVm007731t1,417 235 AAEL002253-PA,182 235 pub16AAEL002253-PA,182 Fringe glycosyltransferase

dmel:NP_524191.1 412 Anofunz4kEVm006079t1,420 286 AFUN007497-PA,134 0 anofun2trinc17253g2t2,420 Fringe glycosyltransferase

;

RefGene RefSize Evigene DiffVB VBgene DiffVO VOgene Name

dmel:NP_523837.2 1637 Aedesg12bEVm000463t1,1692 94 AAEL013729-PB,1598 -2 pub16AAEL013729-PA,1694 Serine/threonine-protein kinase Genghis Khan

dmel:NP_523837.2 1637 Anofunz4kEVm000387t3,1647 757 AFUN009361-PA,890 743 anofun2trinc18651g1t2,904 Serine/threonine-protein kinase Genghis Khan

;

dmel:NP_001261140.1 2989 Aedesg12bEVm000110t1,2955 1152 AAEL009583-PA,1803 0 pub16AAEL010215-PA,2955 Jitterbug, isoform N

dmel:NP_001261140.1 2989 Anofunz4kEVm000112t1,2959 1207 AFUN010466-PA,1752 762 anofun2trinc13712g1t3,2197 Jitterbug, isoform N

;

dmel:NP_730427.1 1747 Aedesg12bEVm000521t1,1791 562 AAEL002345-PA,1229 562 pub16AAEL002345-PA,1229 E3 ubiquitin-protein ligase listerin

dmel:NP_730427.1 1747 Anofunz4kEVm000467t1,1808 0 AFUN004298-PA,1808 1392 anofun2trinc18435g1t1,416 E3 ubiquitin-protein ligase listerin

;

dmel:NP_523358.5 1482 Aedesg12bEVm000773t1,1350 704 AAEL005992-PA,646 189 pub16AAEL001231-PA.3,1161 mind-meld, isoform F

dmel:NP_523358.5 1482 Anofunz4kEVm000692t1,1362 478 AFUN006963-PA,884 253 anofun2trinc16822g4t3,1109 mind-meld, isoform F

;

RefGene RefSize Evigene DiffVB VBgene DiffVO VOgene Name

dmel:NP_732632.1 603 Aedesg12bEVm005042t38,461 -1 AAEL010576-PA,462 -1 pub16AAEL010576-PT,462 Modifier of mdg4

dmel:NP_723354.3 2709 Anofunz4kEVm003923t13,450 113 AFUN002332-PA,337 18 anofun2srro1trinc15259g3t8,432 Modifier of mdg4

;

dmel:NP_001262840.1 1847 Aedesg12bEVm000418t1,1482 262 AAEL006199-PA,1220 262 pub16AAEL006199-PA,1220 Neurexin 1, isoform F

dmel:NP_001262840.1 1847 Anofunz4kEVm000321t2,1444 250 AFUN008763-PA,1194 304 anofun2trinc19031g1t2,1140 Neurexin 1, isoform F

;

dmel:NP_723354.3 2709 Aedesg12bEVm000097t4,2852 445 AAEL006433-PA,2407 445 pub16AAEL006433-PA,2407 piezo, isoform E

dmel:NP_723354.3 2709 Anofunz4kEVm000127t2,2838 1324 AFUN006095-PA,1514 374 anofun2trinc18833g3t2,2464 piezo, isoform E

;

dmel:NP_001138157.1 3722 Aedesg12bEVm000068t1,2969 1651 AAEL003592-PA,1318 1651 pub16AAEL003592-PA,1318 rugose, isoform D

dmel:NP_001138157.1 3722 Anofunz4kEVm000054t2,2692 10 AFUN000209-PA,2682 1042 anofun2trinc18104g3t2,1650 rugose, isoform D

;

RefGene RefSize Evigene DiffVB VBgene DiffVO VOgene Name

dmel:NP_001246211.1 5134 Aedesg12bEVm000012t1,5160 4656 AAEL003837-PA,504 6 pub16AAEL003837-PA.4,5154 ryanodine receptor 44F, isoform I

dmel:NP_001246211.1 5134 Anofunz4kEVm000016t1,5156 3 AFUN006933-PA,5153 3 anofun2trinc19069g1t2,5153 ryanodine receptor 44F, isoform I

;

dmel:NP_725339.1 8805 Aedesg12bEVm000004t11,8455 -93 AAEL018288-PA,8548 -93 pub16AAEL018288-PA,8548 Short stop, isoform H

dmel:NP_725339.1 8805 Anofunz4kEVm000004t2,8514 1329 AFUN003447-PA,7185 7825 anofun2trinc18701g1t2,689 Short stop, isoform H

;

dmel:NP_001260606.1 2707 Aedesg12bEVm000237t3,1789 871 AAEL004494-PA,918 831 pub16AAEL004494-PA.2,958 sickie, isoform I

dmel:NP_001260606.1 2707 Anofunz4kEVm000253t1,1998 1050 AFUN005230-PA,948 1286 anofun2trinc15696g2t1,712 sickie, isoform I

;

dmel:NP_730591.2 2768 Aedesg12bEVm000145t2,2201 2201 0,0 2201 0,0 skuld, isoform E

dmel:NP_730591.2 2768 Anofunz4kEVm000099t1,2198 534 AFUN007494-PA,1664 2 anofun2trinc19036g2t1,2196 skuld, isoform E

;

RefGene RefSize Evigene DiffVB VBgene DiffVO VOgene Name

dmel:NP_001261446.1 2733 Aedesg12bEVm000315t1,2076 877 AAEL004246-PB,1199 877 pub16AAEL004246-PB,1199 still life, isoform J

dmel:NP_001261446.1 2733 Anofunz4kEVm000147t5,2163 508 AFUN004262-PA,1655 1773 anofun2trinc18643g2t4,390 still life, isoform J

;

dmel:NP_001097270.1 3131 Aedesg12bEVm000093t1,2110 179 AAEL002037-PA,1931 179 pub16AAEL002037-PA,1931 Toutatis, isoform E

dmel:NP_001097270.1 3131 Anofunz4kEVm000047t9,1921 444 AFUN003772-PA,1477 907 anofun2trinc16470g4t3,1014 Toutatis, isoform E

;

dmel:NP_728264.1 926 Aedesg12bEVm002528t29,741 10 AAEL013903-PB,731 10 pub16AAEL013903-PB,731 gamma-tubulin ring protein 84, isoform A

dmel:NP_728264.1 926 Anofunz4kEVm002064t1,686 12 AFUN007454-PA,674 0 anofun2trinc16778g1t2,686 gamma-tubulin ring protein 84, isoform A

;

Developed at the Genome Informatics Lab of Indiana University Biology Department