Guide to Daphnia_magna Evigene annotation table columns and GFF mRNA attributes: PredictID (ID in gff mRNA) AAsize : protein aa length TRsize : transcript length Homolog : protein orthology gene ID / bitscore daphnia_hx IDs refer to Daphnia pulex 2010 genes Paralog : protein paralogy gene ID / bitscore Name : homology-derived gene name, /ARP2_G271 gives arthropods.eugenes.org gene cluster ID Flags : gene quality notes, below UnipAC : Uniprot Accessions UnipDE : Uniprot Description Flags 10414 Inparalog paralog stronger than ortholog 13216 Ortholog ortholog stronger than paralog 238 Insplit intron split error 400 Intronerr intron evidence error 58 Join possible join of 2 genes 11556 ncRNA possibly non-coding (or poor gene model) 7717 NostartAA protein missing M start 1262 Transposon likely transposon Note bitscore given for Homolog, Paralog is roughly 2x number of aligned aminos. For example m8AUGapi5s00359g106t1 AAsize=150 Homolog tribolium_TC000621/151 = roughly 50% aligned daphnia_hxJGI_V11_302042/243 = roughly 80% aligned ============================== Examples: PredictID AAsize TRsize Homolog Paralog Flags Location Name UnipAC UnipDE m8AUGapi5s00359g106t1 150 637 tribolium_TC000621/151,daphnia_hxJGI_V11_302042/243 none Ortholog scaffold00359:213191-214000:- signal recognition particle kda protein/ARP2_G2042 P49963 SRP19,Signal recognition particle 19 kDa protein m8AUGep24b_p1s00944g237t1 204 788 daphnia_hxAUG25s17g335t1/274 AUGep24b_p1s00944g238t1/298 Inparalog scaffold00944:1209357-1210519:- glutathione s-transferase/ARP2_G271 P41043 EC=2.5.1.18,Glutathione S-transferase S1 m8PASAgasmbl_40865 227 1624 daphnia_hxAUG26res17g332t1/348 PASAgasmbl_97183/261 ncRNA,Ortholog scaffold00944:1217362-1219662:- glutathione s-transferase/ARP2_G271 P41043 EC=2.5.1.18,Glutathione S-transferase S1 m8AUGep24b_p1s00944g281t1 2078 8374 aphid_m3AUGepir2s880g86t1/2766,daphnia_hxNCBI_GNO_258524/3368 AUGep24bc10359g684t1/144 Ortholog scaffold00944:1373078-1386309:- ferredoxin-dependent glutamate synthase/ARP2_G2419 Q12680 EC=1.4.1.14,Glutamate synthase [NADH]